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National Treasure 3 In Hindi Free Download 720p->DOWNLOADPROJECT SUMMARY During the previous funding period of this award we accomplished the following: (1) established that dendritic cells (DC) can be successfully purified to >99% purity and function as antigen presenting cells (APC); (2) demonstrated that antibody-based magnetic depletion is an efficient method to purify T and B cells; (3) developed methods for the purification of cDC and mDC from mouse tissues and human blood samples; (4) showed that the oral mucosa harbors a unique microanatomical subset of cDC with unique functional attributes; (5) developed a highly sensitive and reproducible quantitative assay to measure cytokine production by DC and have generated?proof-of-concept? data suggesting that this functional assay can distinguish between the abilities of various subsets to deliver antigen to CD4 and CD8 T cells; (6) determined that purified T cells from mice and humans are highly enriched for GFP in the presence of >100,000 transduced cells per inoculum. DC were not enriched; and (7) established that the ability of DC to induce T cell stimulation/proliferation and effector function correlated with antigen capture, TCR triggering and antigen processing. This innovative technology will provide powerful new tools to investigate the role of different DC subsets during mucosal, cutaneous, and non- mucosal infections, which are all associated with major public health problems. We will utilize the latest technological advances to further progress and translate our observations to bench-to-bedside studies in the unique mucosal system that mimics infection with SIV or HIV. Aim 1 will test the hypothesis that the ability of DC to capture antigen correlates with their ability to induce T cell stimulation/proliferation and effector function. Using our novel approach of capturing antigen on carrier beads, captured antigen will be presented on live DC in the presence of labeled antigen-specific T cells to enable direct visualization of antigen capture and processing within DC. We will investigate whether
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