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Chain Kulii Ki Main Kulii 2 Movie In Tamil Download Movie

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al razia and qasida on maine ehtemam download. Tamizazini — Madinali-Azmani— (The sweet songs of home——.Novel selective and specific fluorogenic probes for human imidazole-ring-opened cytochrome P-450 isozymes.
A series of novel 7-methoxy-4-methylcoumarin analogs of a microsomal substrate p-nitrophenol (PNP), which bind selectively to 4alpha- (CYP1A2), 4beta- (CYP1B1) and 2-hydroxyl-microsomal (CYP2E1) forms of human cytochrome P-450 isozymes, have been synthesized and characterized. The 4alpha-, 4beta- and 2-hydroxy-PNP analogs, 7-methoxy-4-methylcoumarin (MMC)-PNP, MMC-4alpha-PNP and MMC-4beta-PNP, were found to be selective fluorescent substrates of respective P-450 isozymes, with apparent K(m) values (mean +/- S.E.) of 1.0 +/- 0.2, 1.0 +/- 0.2 and 1.2 +/- 0.1 microM for CYP1A2, CYP1B1 and CYP2E1, respectively. 7-Methoxy-4-methylcoumarin (MMC) itself does not bind to any isozyme. Determination of the CYP1A2 activity by PNP analog-based fluorescence assay was accurate and precise in comparison with the 7-ethoxyresorufin-O-depentylation assay. The correlation coefficient of the mean values was 0.986 (r = 0.984). Besides, the method was successfully applied to kinetic studies on the respective P-450 isozymes. MMC-4alpha-PNP and MMC-4beta-PNP are potent and selective

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